A liver enzyme that conjugates sulfobromophthalein sodium with glutathione.

The intrahepatic conjugation of sulfobromophthalein and glutathione in the dog.

In 1950 Brauer, Krebs and Pessotti (1) reported that S35-labeled sodium phenoltetrabromophthalein disulfonate (more commonly known as sulfobromophthalein, Bromsulfalein or BSP) may be recovered from the bile of the dog, during constant intravenous infusion, in four fractions which are separable by column chromatography. Similar fractions, accounting for the bulk of BSP excreted and having absor...

The effect of sodium taurocholate on the hepatic metabolism of sulfobromophthalein sodium (BSP). The role of bile flow.

The influence of bile salts on the hepatic metabolism of sulfobromophthalein sodium (BSP) was studied in the perfused rat liver. During sodium taurocholate infusions, hepatic uptake of BSP from plasma was increased and appeared to be related to an enhanced transit of BSP from liver into bile. BSP-glutathione conjugation was not affected by the bile salt infusions, although bile salts inhibited ...

The Importance of Conjugation with Glutathione for Sulfobromophthalein Sodium (bsp) Transfer from Blood to Bile.

Primary active transport of organic anions on bile canalicular membrane in humans.

Biliary excretion of several anionic compounds was examined by assessing their ATP-dependent uptake in bile canalicular membrane vesicles (CMV) prepared from six human liver samples. 2,4-Dinitrophenyl- S-glutathione (DNP-SG), leukotriene C4(LTC4), sulfobromophthalein glutathione (BSP-SG), E3040 glucuronide (E-glu), β-estradiol 17-(β-d-glucuronide) (E2-17G), grepafloxacin glucuronide (GPFXG), pr...

Substrate specificities of rat oatp1 and ntcp: implications for hepatic organic anion uptake.

Transport of a series of 3H-radiolabeled C23, C24, and C27 bile acid derivatives was compared and contrasted in HeLa cell lines stably transfected with rat Na+/taurocholate cotransporting polypeptide (ntcp) or organic anion transporting polypeptide 1 (oatp1) in which expression was under regulation of a zinc-inducible promoter. Similar uptake patterns were observed for both ntcp and oatp1, exce...